Generator

Part:BBa_K150011

Designed by: Pascal Kraemer, Marika Ziesack, Kathrin Nussbaum and Andreas Kuehne   Group: iGEM08_Heidelberg   (2008-10-21)


ColicinE9 Producer Controlled by 3OC6HSL Receiver Device

The constitutive expressed LuxR protein is able to build a complex with the AHL molecule. This complex function as transcription factor for the lux pR promoter and activates by this the colicinE9 production. This activation of the lux pR promoter can additionally lead to expression of the atypical lysis protein by read through of the terminator behind the colE9 gene and therefore to lysis of the cell and colcin release. The colicinE9 immunity protein located on the reverse strand is expressed under a constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1083
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


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Categories
Parameters
None